Custom assay development
qPCR assays built to your targets
Singleplex and multiplex qPCR and RT-qPCR assays, designed and optimized for your targets, dye set and instrument, typically in 3 to 10 weeks.
For Research Use Only. Not for use in diagnostic procedures.
How it works
From target list to finished kit
Scope
Tell us your targets, sample type, instrument and throughput. We confirm feasibility, format and timeline in writing.
Design
Our molecular biologists design primers and probes and assign dyes to fit your instrument's channels.
Optimize
We optimize the assay with a matched SeqOnce™ master mix and build positive controls where needed.
Deliver
You receive a finished kit with documentation, and we support your lab through setup and verification.
Applications
Examples of custom work
Gene expression, genotyping, infectious agents or novel biomarkers. If you can name the target, we can usually design for it.
| Chemistry | qPCR and RT-qPCR |
|---|---|
| Formats | Singleplex and multiplex, in single or multiple tubes |
| Controls | Positive controls included as required |
| Master mix | Optimized SeqOnce™ amplification reagents available alongside the assay |
| Fluorophores | A wide range, including FAM, HEX, Cy5 and ROX |
| Instruments | Developed for the instrument of your choice, including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series |
| Typical timeline | 3 to 10 weeks |
Start a custom assay
Lay out your targets in the panel designer, or send your target list, sample type and instrument. We'll come back with a proposed design, format and timeline.