Women's health and STI

Amur WH Vaginal Microbiota Panel A

34 vaginal microbiota targets and 3 antibiotic resistance genes.

For Research Use Only. Not for use in diagnostic procedures.

Overview

Qualitative multiplex detection of vaginal microbiota and selected antibiotic resistance genes. The panel covers sexually transmitted pathogens, common pathogenic yeasts, bacteria associated with vaginosis and normal Lactobacillus flora.

Targets

Sexually transmitted pathogens

Chlamydia trachomatis
Neisseria gonorrhoeae
Trichomonas vaginalis
Mycoplasma genitalium
Treponema pallidum
Haemophilus ducreyi
Herpes simplex virus 1 (HSV-1)
Herpes simplex virus 2 (HSV-2)
Ureaplasma urealyticum

Vaginosis-associated and other bacteria

Gardnerella vaginalis
Atopobium vaginae
BVAB2
Megasphaera 1
Megasphaera 2
Mobiluncus curtisii
Mobiluncus mulieris
Prevotella bivia
Mycoplasma hominis
Bacteroides fragilis
Group B Streptococcus
Escherichia coli
Enterococcus faecalis
Staphylococcus aureus

Lactobacillus flora

Lactobacillus crispatus
Lactobacillus gasseri
Lactobacillus iners
Lactobacillus jensenii

Yeasts

Candida albicans
Candida auris
Candida glabrata
Candida krusei
Candida lusitaniae
Candida parapsilosis
Candida tropicalis

Resistance genes

mecA (methicillin)
vanA (vancomycin)
vanB (vancomycin)

Internal control

Human RNase P (hRPP)

Specifications

Format	qPCR, qualitative multiplex
Primer and probe mix	10 tubes
Positive controls	4 tubes, included
Fluorophores	FAM, HEX, Cy5, ROX
Sample input	DNA extracted from urine or swab samples using a method that recovers bacterial, viral and fungal DNA. Performance to be verified by the user.
Recommended master mix	SeqOnce™ qPCR Multiplex Amplification Kit 3600, PN 500024. Sold separately.
Compatible instruments	Instruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including: Bio-Rad CFX96 / CFX384 and CFX Opus Roche LightCycler 480 Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage	−20 °C

Ordering

Product	SKU	Reactions
Amur WH Vaginal Microbiota Panel A	500001	48
SeqOnce™ qPCR Multiplex Amplification Kit 3600	500024	48
Panel and master mix bundle	500001 + 500024	48

Product information sheets are available on request.