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Products / Veterinary

Veterinary

Canine Respiratory XL qPCR Panel

11 bacterial and viral targets associated with canine respiratory infection.

For Research Use Only. Not for use in diagnostic procedures.

Overview

Qualitative multiplex RT-qPCR detection of 11 canine respiratory targets, including canine influenza H3N2, in three tubes. When paired with appropriate sampling and extraction methods, the panel is suitable for specimens from veterinary sources. The SPRY1 multi-species internal control monitors extraction and PCR inhibition.

Targets

Bacteria

  • Bordetella bronchiseptica
  • Mycoplasma cynos
  • Streptococcus equi

Viruses

  • Canine adenovirus 2
  • Canine distemper virus
  • Canine herpesvirus 1 (CHV-1)
  • Canine parainfluenza virus
  • Canine pneumovirus
  • Canine respiratory coronavirus (CRCoV)
  • Influenza A H3N2
  • Influenza A (pan influenza)

Internal control

  • SPRY1 multi-species control

Specifications

FormatRT-qPCR, qualitative multiplex
Primer and probe mix3 tubes
Positive controlsCanine Respiratory XL Positive Control, combined (PN 450043) or individual DNA and RNA tubes (PN 450059, 450058), included
FluorophoresFAM, HEX, Cy5, ROX
Sample inputExtracted DNA and RNA from specimens from veterinary sources, using a validated extraction method. Performance to be verified by the user.
Recommended master mixBandit Biosystems RT-qPCR Multiplex Amplification Kit 2250, PN 500044. Sold separately.
Compatible instrumentsInstruments that detect FAM, HEX, Cy5, ROX (or equivalent wavelengths), including:
Bio-Rad CFX96 / CFX384 and CFX Opus
Roche LightCycler 480
Applied Biosystems QuantStudio 3, 5, 6, 7 and 12, and 7500 series
Storage−20 °C

Tube and dye layout

TubeFAMHEXCy5ROX
Tube 1Canine adenovirus 2Bordetella bronchisepticaMycoplasma cynosStreptococcus equi
Tube 2Canine distemper virusCanine herpesvirus 1Canine parainfluenza virusCanine pneumovirus
Tube 3SPRY1 controlInfluenza A H3N2Influenza A (pan)CRCoV

HEX may be replaced by VIC or JOE if your instrument does not have a HEX channel.

Recommended cycling

  • Reverse transcription: 50 °C for 15 minutes
  • Enzyme activation: 95 °C for 2 minutes
  • 40 cycles: 95 °C for 3 seconds, then 59 °C for 30 seconds
  • Collect fluorescence during the 59 °C step

Ordering

ProductSKUReactions
Canine Respiratory XL qPCR Panel500043100
Bandit Biosystems RT-qPCR Multiplex Amplification Kit 2250500044100
Panel and master mix bundle500043 + 500044100

Product information sheets are available on request.